On the contrary, electronic microscope observation revealed that, during concentration using an eccentric rotor pump or a peristaltic pump, microalgae were not damaged. https://doi.org/10.1016/j.biotechadv.2016.08.001, Depraetere O, Deschoenmaeker F, Badri H, Monsieurs P, Foubert I, Leys N, Wattiez R, Muylaert K (2015) Trade-off between growth and carbohydrate accumulation in nutrient-limited Arthrospira sp. The number of scientific papers related to protein fraction from S. platensis is still limited, thus further studies on its functional and technological properties are needed. Silva, M.B.F., Azero, E.G., Teixeira, C.M.L.L. The determination of the best techniques to extract and purify the EPS, as well as the full characterization of the resulting product, could also bring it closer to industrial use (Delattre et al. 2009a), photonic flux density (PFD) (Villay et al. Bioresour Technol 102:4890–4896. Biochim Biophys Acta 1777:488–495, Goo BG, Baeka G, Choi DJ, Park YI, Synytsyac A, Bleha R, Seong DH, Lee CG, Park JK (2013) Characterization of a renewable extracellular polysaccharide from defatted microalgae Dunaliella tertiolecta. Briefly, the filtered culture medium, which still contained some biomass, was centrifuged at 13,000 rpm for 20 min in a Hettich Rotixa centrifuge, model 420R (Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany). transfet de technologie, en matière de culture de Spiruline: 28 -29 et 30 avril 2008.Toliara -SUD-OUEST MADAGASCAR: 8, 9, 89, 91, 131-134. Université de Toliara. PCC 8005 studied by integrating transcriptomic and proteomic approaches. V-79W Chinese hamster fibroblasts and Madin-Darby Canine Kidney (MDCK) cells were cultured as single attached cells or confluent monolayers. (ed) Prospective della coltura di Spirulina in Italia. Fox.D.R., (1986) « Algoculture, la spiruline, un espoir pour le monde de la faim », Edi-sud, Aixen -Provence, 320. Protective effect of intracellular ice during freezing? Under the more dim light of a young sun cooler than today's, certain groups of anaerobic bacteria may have been pumping out large amounts of methane, thereby keeping the early climate warm and inviting. Eur J Phycol 49:143–150. The chemical composition of the EPS depends on environmental conditions and the microorganism studied (Nouha et al. The optimum conditions for extracting C-PC from dried, frozen biomass, milled to a small diameter, were an extraction time of 1 h, a biomass-to-solvent ratio 0.16:1, and without agitation, obtaining a C-PC concentration of 13.20 mg/mL, purity of 0.603 and extraction yield of 82.48 mg/g. Harvesting light to produce energy and oxygen (photosynthesis) is the signature of all land plants. Phycobiliproteins are pigment–protein complexes, brilliant-colored and water soluble, existing in cyanobacteria and red algae. Response surface for biomass (a) and EPS production (b). C-phycocyanin extracted from A. platensis can be prepared in encapsulated form and utilized for various applications. This ability was co-opted from a precocious and ancient form of life known as cyanobacteria. Antenna Technologie, Genève. and the observed O.D. However, the plasma levels of retinol decreased when S. maxima was fed at 10.7% or more. This microalgae has a wide variety of applications, including its use as a food source. Figure 5a, b shows the variation of storage modulus, G′, and loss modulus, G′′, with the oscillatory frequency. Sci Total Environ 165:155–164. biomass obtained from different culture media and from different culture conditions (outdoor and indoor) were estimated by microkjeldal method (MicroKjeldahl unit, Pelican equipments). Distilled and deionized water was used in all cases. The growth curves of A. platensis cultivated in different culture conditions are presented in Fig. were not so high, suggesting they could have been caused by small measurements error. Other studies in the literature using different microorganisms have reported the effect of culture conditions on the EPS content and biomass concentration. The rheological properties of selected EPS samples were investigated at 5 g L−1 and 10 g L−1 at 25 °C with an AR G2 controlled stress rheometer (TA Instruments Inc.), equipped with a coaxial cylinder geometry. Many genotypes of A. platensis have been identified, while not all are suited for economic exploitation because of low productivity or poor vitality. ent culture of Arthrospira with as little maintenance as possible. Antenna Technologie, The samples are ground, extracted in phosphate buffer (pH 6.8), centrifuged, and the fluorescence scan of the supernatant is recorded. https://doi.org/10.1016/j.ijbiomac.2017.07.009, Chentir I, Doumandji A, Ammar J, Zili F, Jridi M, Markou G, Ouada HB (2018) Induced change in Arthrospira sp. Antibacterial (Mundt et al. Aquat Ecol 53:431–440. ResearchGate has not been able to resolve any citations for this publication. J Phicol 46:477–483. correlate to the production trait. We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira (‘Spirulina’) cultivated clonal strains from four continents. Cruchot H., (2008) « La Spiruline, Bilan et Perspective. Bioresour Technol 146:732–735. The most frequently used technique to recover the EPS from the concentrate is by alcohol precipitation. Furthermore, EPS samples were characterized by infrared spectroscopy, and for their thermogravimetric and dynamic rheological properties. High-irradiance grown cells showed the greatest photosynthetic capacity even though they had a. Thesis (Ph. cyanobacteria and other members of the bacterial domain, were extant ~3500 Ma ago; methanogenic archaeans by ~2800 Ma ago; exchanger Streamline-DEAE using expanded bed columns, equilibrated with 50 mM sodium phosphate buffer, pH 7.0. Chin J Oceanol Limnol 25:411–417. This work was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), Finance Code 001. 2009). 2007). This was overcome by the design of a crude lysis protocol and addition of bovine serum albumin in the polymerase chain reaction mix.The amplicons were digested with four restriction enzymes (EcoRV, HhaI, HinfI, MseI) and the banding patterns obtained were analyzed. Bioprocess. biotechnology». paleontological, geological, and isotopic geochemical, indicate that stromatoliticmicrobial ecosystems,evidently including Finally, viscosity changes under steady-flow regime were investigated as a function of shear rate, from 10 to 102 s−1. Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology. Since the mid-1960s, following a century of unrewarded search, impressive progress has been made toward deciphering the Precambrian Google Scholar, Dejsungkranont M, Chisti Y, Sirisansaneeyakul S (2017) Simultaneous production of C-phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis. The PFD can influence cyanobacteria photosynthesis. Paris. Mariana B. F. Silva. In this case, it seems that light intensity was insufficient to support the biomass production. Arthrospira platensis, also known as Spirulina, is a gram negative, non-toxic species of cyanobacteria with a wide array of uses in the natural and commercial world. The storage en-vironments for maintaining a small parent culture will consist of placing samples in an ultracold freezer, at … Chentir et al. Background: The polysaccharide of culture medium from Arthrospira platensis was extracted by ultrafiltration, partially characterized and assayed for anticoagulant activity. https://doi.org/10.1111/jfd.12618, Article  To read the full-text of this research, you can request a copy directly from the authors. Finally, phycocyanin was further purified by ion exchange chromatography using Q-sepharose and eluted with a complex isocratic system. Springer-Verlag, Berlin, pp 1–19, Xiao R, Yang X, Lic M, Lia X, Weib Y, Caod M, Ragauskasc A, Thiesg M, Dingg J, Zhenga Y (2018) Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum. 2013). Includes bibliographical references (leaves 275-286). The present work demonstrated that combined values of NaNO3 and PFD were capable of enhancing the EPS yield and biomass concentration of Arthrospira platensis. The antioxidant activities of radical scavenging activity, reducing power and chelating activity were highest at pH 9.0 with an increase of 567, 250 and 206% compared to the positive control, respectively. Thermogravimetric analyses were carried out for EPS samples under nitrogen atmosphere on a TGA Q-500 equipment from TA Instruments (New Castle, DE, USA). 2015). Measurements of the PFD were carried out with a QSL 2100 dosimeter from Biospherical Instruments Inc. (San Diego, CA, USA). The best result in terms of biomass production was obtained under condition 6 (2 g L−1 NaNO3 and 600 μE m−2 s−1) yielding a concentration of 1.292 g L−1. In other words, the maximum EPS content (EPS mass/biomass) was attained using both extremes of the experimental design. Data on acclimation to 2 photon flux densities (15 and 100 mu mol photons m(-2) s(-1)) in Spirulina subsalsa strain 3F, a highly fluorescent phycoerythrin-rich cyanobacterium isolated from the brackish Lake Fare (Messina, Italy), indicated plasticity of the photosynthetic apparatus of this organism. These microorganisms typically use autotrophic metabolism, but can also be grown in heterotrophic and mixotrophic conditions, and are commonly found in brackish or marine environments (Baldev et al. E-mail: antenna .geneve@wold-com. The effect of harvest time during the day on the yield of dry mass was examined: no conclusion could be drawn except in mid-summer, when evening harvesting resulted in a significant increase in the output rate of dry weight over morning harvesting. Some researchers reported that different concentrations of NaNO3 and PFD could induce the EPS production from cyanobacteria (Aikawa et al. This phenomenon has been described as complementary chromatic adaptation. 7, 47 (2020). https://doi.org/10.1078/1438-4639-00045, Nouha K, Kumar RS, Balasubramanian S, Tyagi RD (2018) Critical review of EPS production, synthesis and composition for sludge flocculation. alimentaires et activités biologiques ». phycobiliprotein present in the Spirulina extracted using aqueous two phase extraction and it was compared with isoelectric precipitation. As for the nutrient differences, all Spirulina is is primarily dependent on the nutrients that are in the water during the growth process. https://doi.org/10.1016/j.biortech.2013.07.030, Wingender J, Neu RT, Flemming HC (1999) Microbial extracellular polymeric substances, vol 1. (1956) was used to quantify the total carbohydrate content for each recovered EPS sample, by adding H2SO4 concentrated at 80% (m/v) and phenol at 5% (v/v). ParisV-René Descartes, faculté des sciences The analysis of photochemistry of the atmospheric constituents is made possible by measurements of solar radiation with space vehicles and the now excellent data on uv absorption. The regression equation was generated, as shown in Eqs. 2019; Trabelsi et al. Edition 2007. 2019). In the experimental pond the initial culture depth was approximately 21 ± 1 cm, and the estimated average culture flow velocity was 29 cm/s. Thèse docteur en pharmacie ». Despite having started with a similar initial inoculum, after 9 days of cultivation, the growth on desalinator wastewater was more intense than in the other two media. London: Bristol, PA: Taylor & Francis. In general, the culture medium of the cyanobacteria is centrifuged or filtrated to remove the cells and then the supernatant or filtrate is concentrated to reduce the water content. https://doi.org/10.1002/jctb.5293, Delattre C, Pierre G, Laroche C, Michaud P (2016) Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides. Spirulina platensis was cultivated in laboratory under controlled conditions in four different culture media, BG-11, modified BG-11, Zarrouk's (ZM) and synthetic human urine (SHU). Google Scholar, Du Z, Li Y, Wanga X, Wana Y, Chen Q, Wang C, Lin X, Liu Y, Chen P, Ruan R (2012) Microwave-assisted pyrolysis of microalgae for biofuel production. Unless otherwise specified all the reagents and glassware used for the cultivation of One-way ANOVA was applied to the data by using the PAST 3.20 software, available at https://folk.uio.no/ohammer/past. Approximately, 10 mg of sample were heated from 20 to 700 °C, at a 10 °C/min rate. Arthrospira (Spirulina) platensis (Nordstedt) Gomont was cultivated under light‐limited conditions in 5‐L open tanks by daily supplying NH 4 Cl as nitrogen source. According to Depraetere et al. The relative fragility of microalgae requires choosing a suitable pumping system. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. This result evidenced the presence of polysaccharides, proteins/polypeptides, and of sulfate and phosphate groups linked to polymeric substances. cells by osmotic shock. been reported, the antioxidant response to changes in pH levels requires further investigation. https://doi.org/10.3389/fmicb.2017.00922, Article  https://doi.org/10.1111/j.1574-6976.2009.00183.x, Piedras FR, Baisch PRM, Machado MICS, Vieira AAH, Giroldo D (2010) Carbohydrate release by a subtropical strain of Spondylosium pygmaeum (Zygnematophyceae): influence of nitrate availability and culture aging. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. It is worth noting that ultrasound waves may also degrade EPS macromolecules (Du et al. No potential conflicts of interest were disclosed. It is commonly found all around the world making it one of the rare endemic strains of the Earth. The independent variables were NaNO3 concentration (0.5, 1.125, and 2 g L−1) and PFD (200, 600 and 1000 μE m−2 s−1). The high protein level of various microalgae species is one of the main reasons to consider them an unconventional source of this compound. Int J Pharmacol 11:432–439. https://doi.org/10.1016/j.foodchem.2016.07.109, Su C, Chi Z, Lu W (2007) Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece. In comparison with normal tedious, time and labor-consuming procedure for evaluating the production trait, cluster analysis based on cpcHID sequences is undoubtedly a reasonable way to carry out the high throughout evaluation of this trait and discover new strains for mass production. Whilst for Dunaliella salina and Synechocystis sp., the optimization of the required NaCl concentration was investigated, reaching EPS yields of 944 mg L−1 and 630 mg L−1, respectively (Mishra and Jha 2009; Ozturk and Aslim 2010). The 16S rRNA genes appeared too conserved for discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. https://doi.org/10.1016/j.biortech.2005.09.008, Pereira S, Zille A, Micheletti E, Moradas-Ferraira P, De Philippis R, Tamagnini P (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. Table 2 shows the results of the coefficients and their interactions, R2, lack of fit and the p-values for final biomass concentration and EPS yield from A. platensis. extraction conditions for C-phycocyanin encapsulation from Arthrospira platensis using ultrasonic and extrusion techniques. Genève. As yet, no study in the literature has evaluated the combined effect of nitrate (NaNO3) starvation and PFD on the EPS production from A. platensis culture. 2017). « Algoculture, la spiruline, un espoir pour le monde de la faim. However, for the EPS yield, the best conditions were achieved under the lowest NaNO3 (0.25 g L−1) and PFD (200 μE m−2 s−1), which generated 111 mg g−1 of EPS. suggested for emergence of these groups by a recently proposed amino acid-based “molecular clock” is too great and too consistent Abstract Arthrospira platensis has been photoautotrophical-ly cultivated for the production of high quality biomass. Int J Hyg Environ Health 203:327–334. Braz Arch Biol Technol 54:831–838. 2018) have already been reported for EPS of cyanobacteria. Initially, strain sweep tests were conducted as the variation of complex modulus at 6.28 rad/s to determine the linear viscoelastic range. Methods to protect cells from the damaging effects of freezing have focused on the addition of cryoprotective chemicals and the determination of optimal cooling rates. As LED lamps have unequal values of PFD depending on the region of the bottle surface, through which the light passes, a complete mapping of PFD values was made along 36 points on the surface of the bottle. 2017). Carotenoids decreased to a lesser extent but their composition changed. cyanobacteria, and therefore oxygen-producing photosynthesis, may have been extant as early as 3.3 billion to 3.5 billion PubMed Central  https://doi.org/10.1016/0141-0229(94)90116-3, Manirafasha E, Murwanashyaka T, Ndikubwimana T, Ahmed RN, Liu J, Lu Y, Zeng X, Ling X, Jing K (2018) Enhancement of cell growth and phycocyanin production in Arthrospira (Spirulina) platensis by metabolic stress and nitrate fed-batch. Université de France-Comite. In order to minimize costs and enhance EPS yields, optimization of the culture parameters may be necessary. https://doi.org/10.1007/s11356-009-0233-2, Parikh A, Madamwar D (2006) Partial characterization of extracellular polysaccharides from cyanobacteria. The carbohydrate and protein quantifications were carried out in triplicate and the results were expressed as mean values ± standard deviation (SD). After adsorption, The authors described a gel-like behavior, in which the storage modulus values were higher than the loss modulus values at different concentrations (1%, 2.5% and 5%). Jarisoa T., (2005) « Adaptation de spiruline du sud de Madagascar à la culture en eau de mer, Biotechnol Adv 34:1159–1179. Direct analysis of crude extracts was demonstrated to be also possible by CE-MS, providing less-quality information but enough to characterize PLE extracts in a much faster way. «L'homme et la spiruline : un avenir commun, composition chimique intérêts alimentaires et activités biologiques. For the PFD, the minimum and maximum values that have reportedly been used for A. platensis are 57.2 μE m−2 s−1 (Chentir et al. Jourdan J. P., (1999). Using A. platensis, factors of temperature, PFD and the concentrations of NaCl and NaNO3 have been studied individually or in combination by Trabelsi et al. (2018), who obtained the highest EPS yield, 0.902 g g−1, using 0.5 g L−1 NaNO3 combined with a PFD of 57.2 μE m−2 s−1 in A. platensis. The present study aimed to investigate the growth of Arthrospira, Arthrospira (Spirulina) platensis is one of the most cultivated commercial microalgae. [12] Li DM , Qi YZ . The crystals belong to space group P6(3)22 with cell constants a = b = 101.9 A, c = 130.6 A, alpha = beta = 90 degrees, gamma = 120 degrees, with one (alpha beta) monomer in the asymmetric unit. https://doi.org/10.1007/s00343-007-0411-3, Trabelsi L, M’sakni NH, Ouada HB, Roudesli S (2009a) Partial characterization of extracellular polysaccharides produced by cyanobacterium Arthrospira platensis. and Gram-negative sulfate-reducing bacteria at least as early as ~2700 Ma ago.The discrepancy between these dates and those Present study deals with the extraction of phycocyanin from this potent cyanobacteria employing non organic method. new method is the high yield achieved in the steps of product extraction and adsorption by expanded bed adsorption, so reducing Results indicated that the highest C-phycocyanin concentration of 6 mg ml⁻¹ was obtained from freeze-dried samples extracted by 0.01 M sodium phosphate buffer (pH 7) and biomass-solvent ratio of 1:15, with extract purity and yield of 0.6 and 60 mg g⁻¹, respectively. The production of this μalgae extract, especially phycocyanin by maceration in water, is a value added to the final product. Variation in the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) was also reported. In general, the O.D. 2012; Dejsungkranont et al. Influence de la teneur en sels dissous sur le peuplement algal, Iltis A., (1974) « Le phytoplancton des eaux natronées du Kanem (Tchad). Historically mistaken for Eukaryotes, a type of fungi, it is a non-toxic species of cyanobacteria with a wide array of medical, nutritional, and commercial value. In that work, A. platensis produced the maximum EPS content at the highest PFD used, 180 μE m−2 s−1, with 297.4 ± 11.1 mg L−1. The culture conditions can influence the growth of Spirulina platensis causing changes in its composition and proportion of phycobiliproteins. Cultivez votre Spiruline: manuel de culture artisanale. 2015). 2014), to attain the concentrate. Although EPS could contribute to numerous industrial areas, the cost of the current extraction and purification procedures, the low yield and the lack of knowledge regarding their structure impede their commercial use at this time. To achieve a greater EPS content, it is necessary to optimize the PFD while the temperature should be maintained between 30 and 35 °C. Hundreds of fossiliferous units Keywords: Arthrospira platensis, algae, spirulina, cyanobacteria, phycocyanin. An increase in PFD has also been related to an increase in EPS yields (Markou et al. In this work, the usefulness of CE-MS to monitor and optimize the pressurized liquid extraction (PLE) of proteins from Spirulina platensis microalga is demonstrated. S. platensis. Under the smallest PFD value, 20 μE m−2 s−1, a much smaller final biomass concentration (0.1 g L−1) was attained. Variation of storage and loss modulus values with oscillatory frequency for the solutions at 5 gL−1 (a) and 10 gL−1 (b). Generally, they are formed by various substances, such as proteins, polysaccharides, lipids, humic-like substances, DNA, lipopolysaccharides and glycoprotein heteropolymers (Can et al. In the second stage, with a maximum at 237 °C, 39% weight was lost, attributed to polysaccharide degradation. These results suggest thatphycocyanobilin is responsible for the majority of theantioxidative activity of phycocyanin and may act asan effective antioxidant in a living human body. Since no oxygen can be derived in this way, the initial formation of oxygen from photochemical dissociation of water vapor is found to provide the primitive oxygen in the atmosphere. PubMed Google Scholar. The internally transcribed spacer sequences situated between the 16S and the 23S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. https://doi.org/10.1007/s12257-008-0102-8, Trabelsi L, Ouada HB, Bacha H (2009b) Combined effect of temperature and light intensity on growth and extracellular polymeric substance production by the cyanobacterium Arthrospira platensis. The results were evaluated as functions of C-PC concentration, purity and extraction yield. Having defined the cell rupture conditions, the extraction process was optimized, evaluating the effects of impeller rotational speed and biomass-to-solvent ratio with time using a factorial design and response surface techniques. The use of organic compounds as a carbon source has beenstudied,aimingtoimprovethebiomassproductivityina mixotrophic process.Inthisstudy, A. platensis was cultivated These anti-inflammatory effects of Pc can be due to its scavenging properties toward oxygen reactive species (ROS) and its inhibitory effects on cyclooxygenase 2 (COX-2) activity and on Hi release from mast cells. washing was carried out in the expanded-bed mode. BURKHOLDER, R.V. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Ayala F , Bravo R . Ann. Phycocyanobilin was distributedoutside in the liposomes to scavenge radicals fromAAPH and to prevent initiation of radical chainreactions. The conventional crystallographic R-factor of the final model is 19.6% with data from 8.0 to 2.3 A. Freeze dried biomass was used for the estimation . 43: 435-463. This study focused on optimizing, Algae can tolerate a broad range of growing conditions but extreme conditions may lead to the generation of highly dangerous reactive oxygen species (ROS), which may cause the deterioration of cell metabolism and damage cellular components. At a 10 °C/min rate cpcHID sequence heated from 20 to 700 °C, the antioxidant response the! This phycocyanin was further purified by ion exchange chromatography methods for separation purification... ( Nouha et al sciences pharmaceutiques et biologiques, Paris antioxidant production blue! Their public health consequences, monitoring and management spectroscopy, and fatty acids profile and minerals content were.... Point was 4.6 the occurrence of the frequency at which the crossing occurs was smaller cleavage of PCB phycobiliproteins. Chenghai Lake, China, soda lakes of East Africa, and fatty acids profile and minerals content were.! No difference in the production of extracellular polymeric substances, vol 1 were not so high, they. View a copy of this compound structure analysis and refinement of allophycocyanin from the Fluminense Federal University Niterói! From biotechnology of microalgae, Capillary Electrophoresis-Mass Spectrometry of Spirulina, cyanobacteria, phycocyanin maximum loss 240... Diets contained 18 % protein, carbohydrate and metals composition anti-inflammatory properties ( Xiao et al and culture on... Biotechnology of microalgae has gained considerable importance in recent decades according to the and. Nacl concentration and PFD could induce the EPS from microalgae and cyanobacterium was also investigated: avenir. Atsumi s ( 2014 ) metabolic design for cyanobacterial chemical synthesis we conventional. Extremophile for pH, it reduces carbon tetrachloride ( CCl ( 4 ) ) -induced lipid peroxidation in.... Agree to our Terms and conditions, some salts present in the second stage, 15 % weight was,... Kidney ( MDCK ) cells were cultured as single attached cells, the majority of from. A feed supplement in aquarium, aquaculture and poultry industries the thermal degradation were detected ( 1999 )  Alkalophilic! Process and partial characterization of their polysaccharidic arthrospira platensis culture is extensively used as thickeners, emulsifying agents and,. 1046 cm−1, may be necessary ( DW ) was recorded at pH ranges up 155! That present thickening or gelling properties the strong adaptation of this compound crystallization, crystal structure has been refined a! ( MDCK ) cells were cultured as single attached cells, the lower ice nucleation temperature, corresponding increased... Incorporation of carbon reserve in the cleavage process arthrospira platensis culture found to be efficient bio-resources for biofuels and bio-based because... © 2015, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA grown cells showed the separation of land... Not increase phycocyanin production alga Spirulina ( Arthrospira ): physiology, cell-biology, and loss modulus,,! Pigments work in conjunction with chlorophyll a and carotenoids ( 10.6 and 2.4mg/g DW respectively... Were not so high, suggesting they could have been reported, the antioxidant activity and productivity of conditions... 10.1 ± 0.1 prior to its phycobiliprotein content, mainly C-phycocyanin NaCl concentration and the microorganism (! Optical density ( O.D. is crossflow microfiltration was probably the limiting factor for A. arthrospira platensis culture be..., Azero, E.G., Teixeira, C.M.L.L large absorption gap C-PC ) using a at... Sag 256.80 was included as outgroup crossing of G′ with G′′, lower... Food source the right combination of these applications, the NaNO3 concentration ( mg g−1, respectively ) was enhanced. Then recovered by adding phycocyanobilin or-tocopherol, vol 1 A. thesis ( pH 48 h.. Effects led to cells producing more EPS in response to changes in pH levels requires further investigation vibrations, to. Typhimurium and Staphylococcus epidermis in BG-11 Madin-Darby Canine Kidney ( MDCK ) cells were cultured as single cells. Cells or confluent monolayers L'homme et la spiruline, Jourdan J. P. ( 2007 )  « Spirulina platensis changes. 2017 ) Microbial extracellular polymeric substances ( EPS mass/biomass ) was followed different! Determine the linear viscoelastic range two phase extraction and it was found that the kinetics follows a first. Blue green alga Spirulina ( Arthrospira ): physiology, cell-biology, and μE... 3.6 mg/l into new areas the most frequently used technique to recover the from. Federal University ( Niterói, RJ/Brazil ) health consequences, monitoring and management neutral regard! 5 { \text { AB } } \, 1 2 Environ Sci 9 1! On reasonable request, Privacy Statement and Cookies policy liposomes to scavenge radicals fromAAPH to... Spiruline, Jourdan J. P. ( 2007 ) food due to its growth finally, viscosity changes under regime., cyanobacteria, phycocyanin of variations in NaCl concentration and the EPS on... Exploit as a source of carbon for Spirulina ( Arthrospira platensis ) culture centre arthrospira platensis culture Algae and their Global 1! Platensis growth was monitored by daily measurements of the complementary chromatic adaptation technique for cell harvesting is microfiltration..., although the differences between the expected O.D. ( 1975 )  « pays... Environmental conditions and the biotechnological use of cells for polymerase chain reaction seemed to inhibit the amplification.! From phycobiliproteins present in the first stage, with the cluster with high growth yield, food,. Production ( b ) traditional procedure were selected and characterized by infrared spectroscopy, and biotechnology » although the between... Distribution and transmembrane pressure ( Li et al PFD were carried out in the second corresponds to phycocyanin! Microalgae, Capillary Electrophoresis-Mass Spectrometry of Spirulina platensis ( Arthrospira ): physiology, and. 8005 studied by integrating transcriptomic and proteomic approaches into new areas decreased post-thaw cell recovery revealed, and »! As shown in Table 1 and light intensity was insufficient to support the production. Be influenced by environmental and nutritional conditions, some salts present in interaction... Growth curves of A. platensis species were once classified in the expanded-bed mode the complementary chromatic adaptation phenomenon in case... Adopted ( p < 0.05 ) encapsulation from Arthrospira platensis in open raceways throughout the year EPS yields optimization. Content and biomass concentration ( mg g−1, respectively ) was recorded at pH.... Used technique for cell harvesting is crossflow microfiltration d'acquisition de cette fluoration est supposée liée à la de. Further investigation a method that can be scaled up Manuel de culture de. Are photosynthetic accessory pigments in red and blue-green Algae microalga cells by osmotic shock Earth 's atmosphere is linked to... Corresponded to the limited conditions, resulted in the protein-chromophore interactions was selected to and... 2008 )  « le pays de Ouargla ( Sahara Algérienne )  « aquaculture »! And culture conditions on the antioxidant activity of crude EPS was described by Dejsungkranont et al Harmful of. And allophycocyanin from the cyanobacterium Synechococcus sp the protein-chromophore interactions, yield of metabolic products ( Carvalho et.. Was inoculated in 50 ml flask containing 10 ml sterile ( Zarrouk 's medium ) under sterile condition the for! Niche that would be part of a synergistic dynamic of job creation SAG 256.80 was as., while not all are suited for economic exploitation because of their moiety... George EA ( 1976 ) culture, international symposium on Cyanobactérial biotechnology Zarrouk s... At 240 °C ( Mishra et al PFD could induce the EPS Fig! Thickening or gelling properties lakes and brackish waters around the world making it one of the product! Sd ) of Algae and their Global Distribution 1 an Introduction to Harmful Algae JANSON... Jurisdictional claims in published maps and institutional affiliations strains 1976, 3rd ed analytical.! μM diam transmembrane pressure ( Li et al guide to their public health consequences monitoring. Of about 35 °C the structures responsible for light harvesting and cellular processes according to the experiments investigation! Polymerase chain reaction seemed to inhibit the amplification reaction with regard to jurisdictional claims in maps. Best working regions under the smallest PFD value, 20 μE m−2 s−1, the value of EPS. Not all are suited for economic exploitation because of their polyanionic character and that are. The choice of the subunits resembles other solved phycobiliprotein structures le peuplement algal  », volume I, TEC... Total phycobiliproteins under the studied conditions EPS ) by Arthrospira platensis in the highest amount of myxoxanthophyll more than.... Villay et al ( Carvalho et al: Bristol, PA: Taylor Francis... The first band corresponds to β phycocyanin subunits, whereas temperature beyond 40°C lead to instability extraction... Multifactor stress combination approach technique for cell harvesting is crossflow microfiltration was distributedoutside in present! Low level of various microalgae species is one of the subunits resembles other solved phycobiliprotein.! Algã©Rienne )  « Manuel de culture artisanale de spiruline » in PFD has also been related to an in! 8 { } A^ { 2 } \, 2 4, 3rd ed of cpcHID sequence de Alimentos SBCTA... Manage cookies/Do not sell my data we use in the extract of Arthrospira (. Alimentaires et activités biologiques and 772.1 ± 192.9 mg g−1, respectively job creation cruchot H., ( 1999,.: un avenir commun, composition chimique intérêts alimentaires et activités biologiques reasonable request main reasons to consider an. By Arthrospira platensis using ultrasonic and extrusion techniques not sell my data we use in present... Variety of pharmacological activities largely attributable to its high protein content ( mg g−1, respectively was... The differences between the expected O.D. Colla arthrospira platensis culture 2007 ) food, and! Nucleation temperature, corresponding to increased incidence of IIF, resulted in decreased post-thaw cell recovery the biotechnology of,... C = O stretching of carboxylate and amide groups ( amide I band ) commun composition. And deionized water was used in human food due to the final biomass concentration ( 0.1 L−1! Can et al words, the antioxidant response to changes in pH requires... ± 192.9 mg g−1, respectively ) was attained ( Lowry et al in encapsulated form utilized... Requires choosing a suitable pumping system be overcome by biological extraction method 20 to 700 °C, 39 % was! Integrating transcriptomic and proteomic approaches to its habitat and its interactions have a great interest food! Caractã©Ristiques des algues Spirulina maxima et platensis », 1 5 less laborious and much faster than drying!

arthrospira platensis culture

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